Research Paper Volume 12, Issue 13 pp 12812—12840

Figure 2. PHLPP2 is a target gene of miR-141 and miR-424. (A) The determination of PHLPP2 mRNA in CCD 841 CoN, HT-29, SW480, Lovo, HCT-116 and SW620 cell lines by RT-qPCR. (B) The expression of PHLPP2 protein in CCD 841 CoN, HT-29, SW480, Lovo, HCT-116, and SW620 cell lines normalized to GAPDH detected by Western blot analysis. (C) The sites of miR-141, miR-424 and miR-32 binding to PHLPP2 predicted by the mircoRNA.org website. (D) The targeting relation between miR-141, miR-424 or miR-32 and PHLPP2 by the dual-luciferase reporter gene assay. (E) The expression of PHLPP2 mRNA influenced by miR-141 and miR-424 in HT-29 cells detected by RT-qPCR. (F) The expression of PHLPP2 protein influenced by miR-141 and miR-424 in HT-29 cells normalized to GAPDH detected by Western blot analysis. * p < 0.05 vs. the NC group; # p < 0.05 vs. the PHLPP2 group; Measurement data in the present Figure are expressed as the mean ± standard deviation, with comparisons among multiple groups performed using One-Way ANOVA; the experiment was repeated three times independently.