Research Paper Volume 12, Issue 13 pp 12812—12840

Figure 9. Overexpression of LINC00402, LINC00461, and SFTA1P enhances the inhibition of PHLPP2 on HT-29 cell proliferation, migration and invasion, and promotion on cell apoptosis. HT-29 cells were transfected with NC, PHLPP2, PHLPP + pcDNA-LINC00402, PHLPP + pcDNA-LINC00461 or PHLPP + pcDNA-SFTA1P. (A) Fluorescence image (200 ×) by EdU labeling showed cell proliferation. (B) Flow cytometry detected cell apoptosis. (C) Scratch test (100 ×) revealed cell migration. (D) Transwell assay (100 ×) suggested cell invasion. (E) Western blot analysis of EMT-related proteins (E-cadherin, N-cadherin, and Vimentin), apoptosis-related proteins (cleaved Caspase-3 and Bax) and Akt3-p27 pathway-related proteins (Akt1, Akt3, and p27) in HT-29 cells normalized to GAPDH. * p < 0.05 vs. the NC group; # p < 0.05 vs. the PHLPP2 group. Measurement data in this Figure were expressed as the mean ± standard deviation, with comparisons among multiple groups conducted by One-Way ANOVA; the experiment was repeated three times independently.