Research Paper Volume 12, Issue 18 pp 17902—17920
Long noncoding RNA SBF2-AS1 contributes to the growth and metastatic phenotypes of NSCLC via regulating miR-338-3p/ADAM17 axis
Figure 2. Knockdown of SBF2-AS1 suppresses NSCLC cell proliferation, migration and invasion. (A) qRT-PCR analysis of SBF2-AS1 expression in BEAS-2B and NSCLC cell lines (H1650, A549 and H1975). **P<0.01 compared with BEAS-2B. (B) qRT-PCR analysis of SBF2-AS1 expression in A549 or H1975 cell transfected with si-Con or si-SBF2-AS1. (C) Cell proliferation capacity was analyzed by CCK-8 assay. (D) Cell proliferation capacity was analyzed by colony formation assay. (E) The migration capability of A549 or H1975 cell transfected with si-Con or si-SBF2-AS1 was analyzed by wound-healing assay. (F) The invasion capability of A549 or H1975 cell transfected with si-Con or si-SBF2-AS1 was analyzed by Transwell assay. (G) The expression of si-SBF2-AS1 in A549 and H1975 cell decreased secreted MMP-2/9 protein levels, as shown by ELISA. The data are presented as the mean ± SD. All in vitro data are representative of three independent experiments. **P<0.01 compared with control.