Research Paper Volume 12, Issue 18 pp 17902—17920

Figure 4. Knockdown of SBF2-AS1 suppresses NSCLC cell proliferation and invasion by targeting miR-338-3p. (A) qRT-PCR analysis of miR-338-3p expression in human bronchial epithelial cell BEAS-2B and NSCLC cell lines (A549, H1650 and H1975). ^**P<0.01 compared with BEAS-2B. (B) A549 or H1975 cell was transfected with si-Con, si-SBF2-AS1 or si-SBF2-AS1 and miR-338-3p inhibitor. The expression levels of miR-338-3p in different groups were analyzed by qRT-PCR. (C) Cell proliferation activity of A549 or H1975 cell was assessed by CCK-8 assay. (D) Cell proliferation activity of A549 or H1975 cell was assessed by cell colony formation assay. (E) Cell migration ability of A549 or H1975 cell in different groups was analyzed by wound healing assay. (F) Cell invasion ability of A549 or H1975 cell in different groups was analyzed by Transwell invasion assay. The data are presented as the mean ± SD. All in vitro data are representative of three independent experiments. ^**P<0.01 compared with si-Con, ^##P<0.01 compared with si-SBF2-AS1.