Research Paper Volume 12, Issue 13 pp 12943—12959

Nrf2 inhibits ferroptosis and protects against acute lung injury due to intestinal ischemia reperfusion via regulating SLC7A11 and HO-1


Figure 1. IIR induces ferroptosis in type II alveolar epithelial cells. (A) The degree of pulmonary oedema continued to increase with the extension of reperfusion time. (B) Representative transmission electron micrographs of the ultrastructure of lung tissues. Scale bars: 1 μm. (C) HE staining of the lung tissues of mice following IIR, IIR + Fe, and IIR + Fer-1. Scale bars: 200 μm. (D) The lung pathological damage score showed an addition and reduction after Fe and Fer-1 administration, respectively. (E) The wet to dry ratio of the lung tissue shown in each group. (F) The level of lipid peroxide MDA in each group. (G) The GSH level in each group. The error bars represent the standard error from three replicates. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 between the groups; *compared with the sham group; # compared with the IIR group.