Priority Research Paper Volume 12, Issue 10 pp 8766—8789

Figure 2. Expression of UCP1 in adipose tissue of Snell Dwarf mice (dw). (A) RNA was isolated from brown fat, mesenteric, inguinal and perigonadal adipose tissues of 24-week-old littermate control (WT) mice and Snell Dwarf mice (dw). mRNA levels of UCP1 were measured by qRT-PCR. Data (mean ± SEM; n = 4) were normalized by the amount of GAPDH mRNA and expressed relative to the corresponding male WT value. *P < 0.05 versus WT. (B) Cell lysate was prepared from brown fat, inguinal and perigonadal adipose tissues of 24-week-old WT and dw mice, and protein levels of UCP1 were measured by western blotting. Representative gel images are shown. (C) Relative protein expression was normalized to β-actin levels. Values are mean ±SEM (n = 4).