Research Paper Volume 12, Issue 20 pp 20069—20084

FAM181A-AS1 knockdown inhibits glioma cell proliferation and colony formation. (A) QRT-PCR analysis shows FAM181A-AS1 levels in the four glioma cell lines, namely, LN229, A172, U87 and U251, and the control normal human astrocyte (NHA) cell line. (B) QRT-PCR analysis shows FAM181A-AS1 levels in si-NC- and si-FAM181A-AS1-transfected LN229 and A172 cell lines. (C, D) CCK-8 and EdU assay results show proliferation of si-NC- and si-FAM181A-AS1-transfected LN229 and A172 cell lines. (E) Colony formation assay results show the numbers of colonies formed by si-NC- and si-FAM181A-AS1-transfected LN229 and A172 cells. (F) Western blot analysis shows the levels of PCNA protein in si-NC- and si-FAM181A-AS1-transfected LN229 and A172 cell lines. Note: **P<0.01, ***P<0.001; data are represented as means ± SD.