Research Paper Volume 12, Issue 20 pp 20069—20084

ZRANB2 is a downstream target gene of miR-129-5p. (A) RNA22, miRanda and PicTar analyses results show identification of 8 potential miR-129-5p target genes, namely, ZNF646, RBPJ, CAMTA1, FBXW7, ZRANB2, L1CAM, PDS5A and ZFHX3. (B) Diagrammatic representation shows miR-129-5p binding sites in the WT and MUT 3'UTR of ZRANB2. (C) Dual luciferase reporter assay results show relative luciferase activity in U87 and U251 cell lines transfected with miR-129-5p mimic and plasmid vectors carrying WT or MUT 3′UTR of ZRANB2. (D) QRT-PCR results show ZRANB2 mRNA levels in glioma cell lines and the control NHA cell line. (E, F) QRT-PCR and Western blot analyses show ZRANB2 mRNA and protein levels, respectively, in FAM181A-AS1-overexpressing U87 and U251 cell lines transfected with miR-NC or miR-129-5p mimic. Note: **P<0.01, ***P<0.001; ns, not statistically significant; data are represented as means ± SD.