Research Paper Volume 12, Issue 13 pp 13038—13058

Figure 3. circMET may function as a sponge for miR-145-5p. (A) CircRIP experiments were performed in A549 cells using a circMET probe and NC probe. (B) RIP assays were performed on extracts from NSCLC cells using an antibody against AGO2. (C) The putative miR-145-5p binding sites with respect to circMET were predicated by StarBase v3.0. (D) The luciferase activity of pLG3-circMET in HEK-293T cells after cotransfection with miR-145-5p. (E) The level of circMET in the streptavidin-captured fractions from the NSCLC cell lysates after transfection with biotinylated miR-145-5p or control RNA (NC). cANRIL was used as a NC. (F) Colocalization between miR-145-5p and circMET was observed by the double FISH assay. Nuclei were stained with DAPI. Scale bar, 20um. The data was represented as the mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. NS: no significant.