Research Paper Volume 12, Issue 13 pp 13038—13058

Figure 4. circMET regulates miR-145-5p and targets the CXCL3 axis. (A) and (B) RT-qPCR analyses the mRNA levels of miR-145-5p expression after overexpressing circMET in NSCLC A549 and 95D cells. GAPDH was used as a loading control. (C) The putative miR-145-5p binding site in the 3’UTR of CXCL3 was predicated by StarBase v3.0. (D) the luciferase activity of pLG3-CXCL3 in HEK-293T cells after cotransfection with miR-145-5p. (E) and (F) RT-qPCR analyses CXCL3 expression after overexpressing circMET in NSCLC A549 and 95D cells. GAPDH was used as a control for loading. (G) and (H) CircMET significantly promoted the mRNA and protein levels of CXCL3 expression, whereas the effect was retarded after upregulation of miR-145-5p. The data are represented as the mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001. NS: no significant.