Research Paper Volume 12, Issue 13 pp 13128—13146

Long-term low-dose ethanol intake improves healthspan and resists high-fat diet-induced obesity in mice


Figure 5. Low-dose ethanol intake increased insulin sensitivity of HFD mice. (AD) Serum Leptin level, insulin level, IGF-1 level, and adiponectin level of four treatment regimens at week 44. (n=9 per group). (E) Plasma levels of glucose after intraperitoneal insulin injection (ITT) and area under curve (AUC) (n=9 per group). (FG) Plasma levels of glucose and insulin measured after oral glucose tolerance test (OGTT) (n=7-10 per group). The tests were performed with 44-week-old mice under 12-weeks HFD/normal diet treatment. For oral glucose tolerance test (OGTT), mice were fasted overnight and gavaged with 2 g/kg of glucose. Insulin tolerance tests (ITT) were performed in nonfasted mice by IP injection of 1.5 IU/kg of insulin. Data are presented as mean ± SEM. *, P< 0.05, **, P< 0.01, ***, P< 0.001.