Research Paper Volume 12, Issue 13 pp 13255—13280

Figure 6. miR-1271-5p targets mTOR in PCa cells. (A) Target genes of miR-1271-5p were predicted by Starbase 2.0. (B, C) qRT-PCR analysis of the expression of target genes in PC-3 and DU145 cells treated with the miR-1271-5p mimic. (D) RegRNA 2.0 predicted the binding site of miR-1271-5p on circMBOAT2. (E) Wild type (wt) and mutated (mut) sequences of the predicted binding site between the MTOR 3’UTR and miR-1271-5p. (F) Dual-luciferase reporter assays showing the binding properties of MTOR and miR-1271-5p. Renilla luciferase (Rluc) activity was normalized to firefly luciferase activity. (G) Western blot analysis of mTOR expression in PC-3 and DU145 cells treated with miR-1271-5p. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.