Research Paper Volume 12, Issue 13 pp 13255—13280

Figure 7. circMBOAT2 upregulates mTOR expression by sponging miR-1271-5p. (A–C) Representative images and quantification of colony formation assays in PC-3 and DU145 cells treated with miR-1271-5p mimic or mimic NC, transfected with vector or circMBOAT2 plasmid. (D–H) Representative images and quantification of transwell assays in PC-3 and DU145 cells treated with miR-1271-5p mimic or mimic NC, transfected with vector or circMBOAT2 plasmid. Scale bars: 100 μm. (I–K) Representative images and quantification of wound healing assays in PC-3 and DU145 cells treated with miR-1271-5p mimic or mimic NC, transfected with vector or circMBOAT2 plasmid. Scale bars: 200 μm. (L) Western blot analysis of expression of mTOR and its substrates in PC-3 and DU145 cells treated with miR-1271-5p mimic or mimic NC, transfected with vector or circMBOAT2 plasmid. Data are displayed as mean ± SD. *p < 0.05; **p < 0.01.