Research Paper Volume 12, Issue 13 pp 13354—13364

Figure 2. IL-22 stimulates proliferation of breast cancer cells in vitro. (A, B) 4T1, MCF7 and MDA-MB-231 cells were cultured with different doses of rhIL-22 or rmIL-22 for 24 h. Cells cultured without IL-22 were used as control. (A) Cell proliferation was measured with CCK-8 assay (n=6). *, p < 0.05. (B) Western blot was applied to analyze levels of indicated proteins. Experiment was repeated twice. Intensities of phospho-proteins were analyzed using ImageJ software, and were normalized to relative total proteins. *, p < 0.05. (C) 4T1, MCF7 and MDA-MB-231 cells were cultured with IL-22 (100 ng/ml) in the presence of different doses of stattic for 24 h. Cells cultured without stattic were used as control. Cell proliferation was measured with CCK-8 assay (n=6). *, p < 0.05, compared with control in IL-22 (100 ng/ml) + stattic group; n.s., not significant, compared with control in PBS + stattic group. Data are mean ± SD, compared using unpaired t test or one-way ANOVA test.