Research Paper Volume 12, Issue 13 pp 13463—13476

Figure 5. The effect of SOD2 gene knock-down on mtROS-JNK signaling in BPV-induced neuron oxidative stress. Con: cells transfected with silencer negative control siRNA; siSOD2: cells transfected with SOD2 siRNA. BPV: cells transfected with silencer negative control siRNA and treated with 2.0 mM BPV for 60 min; siSOD2+ BPV: cells transfected with SOD2 siRNA and treated with 2.0 mM BPV for 60 min. (A, B) the western blot analysis showed SOD2 in cells transfected with siRNA and treated with BPV; (C, D) mtROS was monitored by flow cytometry which showed the effect of knockdown SOD2 on mtROS in cells transfected with siRNA and treated with BPV. (E, F) the western blot analysis showed the activation of JNK signaling in cells transfected with SOD2 siRNA and treated with BPV. Values are the mean± SEM of n = 3, *: P< 0.05.