Research Paper Volume 12, Issue 18 pp 17930—17947
Modulating redox homeostasis and cellular reprogramming through inhibited methylenetetrahydrofolate dehydrogenase 2 enzymatic activities in lung cancer
Figure 2. MTHFD2 knockdown on growth and proliferation potential of lung cancer. (A) Relative gene expression of MTHFD2 in knockdown group compared to vector control. *** indicate p<0.001 using one-way ANOVA. (B) The MTHFD2 protein expression of vector control group and MTHFD2-knockdown group, where β-actin as loading control. (C) MTT assay-dependent cell viabilities of MTHFD2-knockdown groups of A549, H1299 and H441 compared to vector control. (D) The morphology and quantification of formed colonies of vector control and MTHFD2-knockdown groups of A549, H1299 and H441 cell lines by soft agar assay. * and ** indicate p<0.05 and p<0.01, respectively using one-way ANOVA, compared to shVector group.