Research Paper Volume 12, Issue 18 pp 17930—17947
Modulating redox homeostasis and cellular reprogramming through inhibited methylenetetrahydrofolate dehydrogenase 2 enzymatic activities in lung cancer
Figure 3. MTHFD2 knockdown and assessment of lung cancer stemness through sphere formation assay. (A) Representative protein expression of MTHFD2 and HIF-1α in adherent non-sphere forming cells (Ctrl) and sphere cells (Sphere) of parental cell lines, β-actin was used as loading control. (B) Bright-field images of primary and secondary tumor spheres (at 4X and 10X magnifications) and (C) their relative quantification of vector control and MTHFD2-knockdown cell lines, A549, H1299 and H441. * and ** indicate p<0.05 and p<0.01, respectively using paired t-test.