Research Paper Volume 12, Issue 13 pp 13594—13617
The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing αB-crystallin expression
Figure 1. Treatment of rat lens with 40 mU GO caused apoptosis of lens epithelial cells. (A) Dynamic H2O2 concentration generated from 40mU glucose oxidase (GO) in the M199 medium in which rat lenses were cultured in 10-cm culture dish with 30 ml medium. (B) Dynamic changes of free thiol levels in rat lens epithelial cells under 40 mU GO treatment. (C) Live/dead assays to reveal time-dependent apoptosis of rat lens epithelial cells under treatment of 40 mU GO. Green fluorescence represents live cells as detected by Calcein-AM, and red fluorescence detected by EthD-1 refers to dead cells. (D) Apoptotic rate of rat lens epithelial cells under 40 mU GO treatment. All experiments were repeated three times. Error bar represents standard deviation, N=3.