Research Paper Volume 12, Issue 13 pp 13594—13617
The transcription factor CREB acts as an important regulator mediating oxidative stress-induced apoptosis by suppressing αB-crystallin expression
Figure 2. GO-induced apoptosis of rat lens epithelial cells is derived from downregulated expression of αB-crystallin caused by upregulation of CREB. (A) Western blot analysis of αB-crystallin in rat lens epithelium with 40mU glucose oxidase treated from 0 to 180 minutes. (B) Semi-quantification of the western blot results in (A). (C) Western blot analysis of total CREB (T-CREB) in rat lens epithelium with 40mU glucose oxidase treated from 0 to 180 minutes. (D) Semi-quantification of the western blot results in (C). Note the reverse relationship between expression of αB-crystallin with that of total CREB expression. All experiments were repeated three times. Error bar represents standard deviation, N=3. * p<0.05; ** p<0.01; NS, statistically not significant.