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Research Paper Volume 12, Issue 16 pp 16062—16071
Synergistic effect of electric stimulation and mesenchymal stem cells against Parkinson's disease
Figure 1. Preparation of genetically labeled MSCs. (A) Schematic of lentivirus carrying luciferase and RFP reporters with an 2A connector under the control of a CMV promoter. (B) Flow cytometry for transduced MSCs (MSC-LR) for RFP. (C) MSCs-LR in cell culture, showing RFP label and nuclei by DAPI staining. (D) Bioluminescence was detected in MSCs-LR cells after luciferin exposure. (E) Osteocyte-, adipocyte- and chondrocyte- differentiation of MSCs-LR and control MSCs, validated by Von Kossa staining, Oil red O staining and Alcian blue staining, respectively. *p<0.05. N=5. Scale bar in panel C is 20μm and in panel D is 100μm.