Research Paper Volume 12, Issue 13 pp 13647—13667

Figure 5. MiR-146b-5p is downregulated in F-GSC/MSCs and its overexpression inhibits F-GSC/MSC proliferation, invasion and migration. (A) Hierarchical clustering demonstrated distinguishable miRNA expression profiles in MSC and F GSC/MSC microarray data. (B) qPCR verified miR-146b-5p expression was the lowest among downregulated miRNAs in F-GSC/MSCs. miR-146b-5p expression in (C) F-GSC/MSs was lower than in MSC-GFPs or SU4-RFPs. (D) miR-146b-5p levels in F-GSC/MSs were upregulated by transfection of miR-146b-5p mimics. (E) Proliferation of F GSC/MSCs transfected with miR-146b-5p mimic or negative control was measured with CCK8 assays. (F and G) Colony formation assays with F GSC/MSCs transfected with miR-146b-5p mimic or negative control. (H and I) Effect of miR-146b-5p upregulation on F-GSC/MSC proliferation was determined using EdU assays. (J and K) Effect of miR-146b-5p upregulation on invasiveness of F GSC/MSCs was determined using Matrigel transwell assays. (L and M) Effect of miR-146b-5p on F-GSC/MSC migration was evaluated in wound-healing assays.