Figure 2. Gas6/Axl was involved in the anti-senescence effect of testosterone on vascular cell senescence. (A) The relative telomere length was detected by real-time PCR. (B) Representative Western blot and analysis of p16INK4a and p21Cip1. (C): Representative b-galactosidase staining with the descending aorta from the mouse (scale bar: 20μm). (D): Analysis of the relative dyeing rate. Data are mean ± SD; **P < 0.01 and ***P < 0.001 vs. WT young group; †P < 0.05 and ††P < 0.01 vs. WT old group; &&P < 0.01 and &&&P < 0.001 vs. Axl-/- young group; A-B: n = 3 for each group, C-D: n = 5 for each group.