Research Paper Volume 12, Issue 16 pp 16111—16125

Figure 5. Testosterone ameliorated vascular fibrosis in aging-related arterial stiffness. (A1-4) Representative H&E staining, Masson trichrome staining, Picrosirius red staining and Verhoeff Van Gieson (scale bar: 20μm). (B–E) Quantitative analyses of collagen content, elastin content, collagen content/elastin content and average aortic wall architecture score. (F) Immunohistochemical staining for collagen I and III (brown staining considered positive staining; scale bar: 20μm). (G) Representative Western blots of collagen I and III. (H) Western blot analyses of collagen I and III. Data are mean ± SD; ^*P < 0.05 and ^**P < 0.01 vs. WT young group; ^†P < 0.05 and ^††P < 0.01 vs. WT old group; ^&P < 0.05, ^&&P < 0.01 and ^&&&P < 0.001 vs. Axl^-/- young group; ^#P<0.05 vs. Axl^-/- old group; A-F: n = 5 for each group, G-H: n = 3 for each group.