Research Paper Volume 12, Issue 16 pp 16142—16154

Figure 2. LAIR-1 inhibits SKOV3 cells growth and promotes cell apoptosis in vitro. (A) SKOV3 cells were transfected with control or LAIR-1 overexpressed lentivirus, the efficiency of LAIR-1 overexpression was examined by fluorescence microscope. (B, C) qRT-PCR and western blot were also used to further confirm the efficiency of LAIR-1 overexpression in SKOV3 cells. GAPDH was used as a loading control. (D–F) The cell growth was measured by cell proliferation assay and colony formation assay in control and LAIR-1 overexpressed SKOV3 cells. (G, H) Cell migration ability was assessed by transwell migration assay in control and LAIR-1 overexpressed SKOV3 cells. (I, J) Flow cytometry analysis of cell apoptosis in control and LAIR-1 overexpressed SKOV3 cells. Cells were collected and stained with Annexin V-fluorescein isothiocyanate (FITC) and PI. Data are presented as mean ± SD.