Figure 2. Bioinformatic analyses of SCD1. (A–C) Receiver operating characteristic (ROC) curve at 1, 3 and 5 years according to SCD1 gene expression in the TCGA-STAD database. (D–F) The calibration curve for predicting overall survival at 1-, 3- and 5-years in the TCGA-STAD database. (G) Representative images from gastric cancer tissue stained with SCD1, the scale bar, 100 μm and 50 μm, respectively. (H) Endoplasmic reticulum of gastric cancer cells were isolated, proteins were separated by SDS-PAGE and evaluated by immunoblots. (I) The alteration frequency of SCD1 were determined by using the cBioportal. (J) Screenshot of SCD1 mutation frequencies. (K) The Venn diagram illustrated the common correlated factors of SCD1 identified via cBioportal, Gepia and MEM analysis tools. (L) The correlations between SCD1 and correlated factors mRNA expression levels in human gastric cancer tissues (TCGA, n=375).