Research Paper Volume 12, Issue 15 pp 15374—15391

Figure 3. Oncogenic function of SCD1 in gastric cancer cells. (A) The KEGG pathways and GO terms participated by SCD1 and related factors with P value < 0.05. (B) The KEGG pathways and GO terms identified via gene set enrichment analysis of tissues with high and low SCD1 expression levels. (C) The proteins participated in “DNA replication”, “cell cycle”, “cell cycle G1-S phase transition” and “cyclin dependent protein serine threonine kinase regulator activity”, anti-ferroptosis markers as well as Wnt/β-catenin and Hippo signaling pathways were analyzed using western blotting with the indicated antibodies. GAPDH was used as the internal protein loading control. Each experiment was examined in triplicates. (D, E) SCD1 promoted proliferation of gastric cancer cells. (F–I) SCD1 promoted colony formation of gastric cancer cells. Each experiment was examined in triplicate. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P <0.0001, respectively.