Research Paper Volume 12, Issue 17 pp 16936—16950

Figure 1. Establishment, identification and biological characteristics of radiotherapy resistant strains of nasopharyngeal cancer cells. (A) Cell morphology identification. CNE-2R and 6-10BR cell lines were established from CNE-2 and 6-10B, respectively. The cumulative dose of radiation of CNE-2R and 6-10BR reached to 80 and 76Gy, respectively. Under the optical microscope, the morphology of the cell line was obviously changed. (B and C) CNE-2R, 6-10BR and their parental cells were subjected to a sphere formation assay. The sphere numbers were determined after seven days for the first generation (G1) and seven days after seeding for G2. Treatment with SCF was repeated when the cells were passaged. The data are mean±SD of two independent experiments. (D and E) Radiosensitivity detection assay showed that the sensitivity of CNE-2R and 6-10BR cells was lower than that of parental cells. (F and G) Cell proliferation assay showed that the proliferation of CNE-2R and 6-10BR cells was slower than that of parental cells.