Research Paper Volume 12, Issue 17 pp 16936—16950

Figure 3. FAM133B-2 is a target of miR-34a-5p in nasopharyngeal cancer cells. The relative miR-34a-5p level (fold) in CNE-2R and 6-10BR cells versus CNE-2 and 6-10B cells measured by both miR-omic and qRT-PCR analyses is shown in a table (A) and those measured by qRT-PCR are shown in a plot (B). “-” indicates no detection in the omic analysis. (C) Luciferase reporter constructs: WT and MUT FAM133B-2 in the miR-34a-5p-binding sites were inserted into psiCHECK-2 vector. The red region is the binding site. The FAM133B-2 site is predicted to be a target of miR-34a-5p. One seed sequence mutant of miR-34a-5p was shown below. (D and E)The relative luciferase activities (fold) of the reporter with the wild-type (WT) or mutant-type (MUT) FAM133B-2-UTR or without the UTR (NC) were determined in the nasopharyngeal cancer cells transfected with the miR-34a-5p mimic (in CNE-2R) or antagomiR (in CNE-2). (F and G) MiR-34a-5p mimic (5PM)-transfected CNE-2R and 6-10BR increases survival fraction versus the negative control (NC) cells. (H and I) MiR-34a-5p antagomiR (5PA)-transfected CNE-2 and 6-10B decreases NC cells survival fraction versus the negative control (NC) cells.