Research Paper Volume 12, Issue 17 pp 16759—16774

Figure 3. HDAC4 knockdown decreases TET2 expression. (A) Nomo-1 cells were transduced with siRNAs targeting indicated HDAC genes, and TET2 expression was determined by RT-qPCR. (B, C) HDAC4 knockdown using lentivirus to deliver shRNA to MDS-L and Nomo-1 cells. TET2 expression was determined by RT-qPCR (B) or Western blot (C). (D) Total BM and c-kit⁺ cells were harvested from HDAC4 WT or KO mice, and TET2 protein levels were determined by Western blot. (E) MDS-L and Nomo-1 cells were treated with 5 μM LMK-235 or 1 μM MS-275 for 24 hours, and TET2 expression was determined by RT-qPCR. (F) MDS-L cells treated with LMK-235 or MS-275 were analyzed by Western blotting for TET2 expression. H3K27Ac served as a positive control.