Research Paper Volume 12, Issue 18 pp 18073—18083

Figure 3. RIPK1 was the direct target of miR-141-3p and aggravated LPS-induced Caco-2 cell injury. (A) The predicted binding sites between miR-141-3p and RIPK1, and Luciferase reporter gene assay was performed to test the interaction between miR-141-3p and RIPK1. (B) The protein level of RIPK1 in miR-141-3p mimics-transfected Caco-2 cells. (C) The expression of RIPK1 in Caco-2 cells with RIPK1 overexpression and knockdown. Cell viability of LPS-treated Caco-2 cells with RIPK1 overexpression (D) or knockdown (E). The expression of IL-6 (F) and TNF-α (G) in LPS-treated Caco-2 cells with RIPK1 overexpression. The mRNA level of IL-6 (H) and TNF-α (I) in LPS-treated Caco-2 cells with RIPK1 siRNA transfection. ^*p<0.05, ^**p<0.01, ^***p<0.001.