Research Paper Volume 12, Issue 18 pp 18073—18083
MiR-141-3p ameliorates RIPK1-mediated necroptosis of intestinal epithelial cells in necrotizing enterocolitis
Figure 4. LPS induced RIPK1-mediated necroptosis of Caco-2 cells. (A) PI positive staining of LPS-treated Caco-2 cells with pcDNA3.1-RIPK1 transfection or RIPK1 siRNA transfection. (B) The protein level of RIPK1, p-RIPK3, RIPK3, p-MLKL and MLKL in LPS-treated Caco-2 cells. (C) IP assay was conducted to determine the interaction between RIPK1 and RIPK3 in LPS-treated Caco-2 cells. Scale bar = 20 μm.