Research Paper Volume 12, Issue 18 pp 18084—18098

Figure 6. Regulation of RAI14 in EC proliferation depends on the STAT3 pathway. The STAT3 inhibitor, AG490, was dissolved in DMSO. TE-14 cells that were transfected with the RAI14-vector or RAI14 were treated with DMSO (Vehicle) or AG490. (A) Protein levels of RAI14, p-STAT3, total STAT3, Mcl-1, cyclin D1, and cleaved caspase-3 were determined by western blotting. GAPDH was used as the internal control. (B) The proliferation of cells was assessed using the CCK-8 assay. (C) Flow cytometry scatter plot of apoptosis. (D) Flow chart depicting cell cycle distribution that was assessed by flow cytometry. (E) Apoptosis rate. (F) Proportion of cells in each cell-cycle phase. *P < 0.05 versus the respective control group.