Research Paper Volume 12, Issue 18 pp 18127—18136

Figure 2. GHR blockage increased sorafenib inhibition of HCC cell viability. (A) MTT assay tested the IC50 values of sorafenib on HepG2 and Huh7 cells with the presence or absence of GHR. In HepG2 cells with siGHR, IC50 values is 0.4009 μM, whereas on control cells the value is 5.265 μM. In Huh7 cells with siGHR, IC50 values is 0.7053 μM, and on control cells the value is 4.722 μM; (B) The results of colony formation assay showed that GHR silence significantly inhibited cell proliferation in HCC cells treated with different concentration of sorafenib (0, 5 μM or 10 μM); (C) The percentage of cell viability was normalized. All data shown represented the mean ± SEM. ***P < 0.001, compared with control groups.