Research Paper Volume 12, Issue 17 pp 17079—17098

Figure 7. UNC5B knockdown inhibited the proliferation and metastatic capacities of breast cancer cells. (A) Quantitative PCR analysis of UNC5B expression in T-47D and MDA-MB-231 cells after UNC5B-shRNA introduction. (B) Western blot analysis of UNC5B expression in T-47D and MDA-MB-231 cells after UNC5B-shRNA introduction. (C) The proliferation of T-47D and MDA-MB-231 cells after UNC5B knockdown. (D, E) Representative images and quantified results of colonies formation in T-47D and MDA-MB-231 cells after UNC5B knockdown. (F, G) Representative images and quantified results of transwell migration assay in T-47D and MDA-MB-231 cells after UNC5B knockdown. (H, I) Representative images and quantified results of matrigel-treated transwell invasion assay in T-47D and MDA-MB-231 cells after UNC5B knockdown. Data represent three independent experiments. Data are expressed as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.