Research Paper Volume 12, Issue 17 pp 17099—17113

Figure 4. SIRT6-deficiency leads to malformed spermatozoa with impaired acrosomes. (A) Immunofluorescence staining of PNA (green) and DAPI (blue) in Sirt6^+/+ and Sirt6^-/- spermatids. The acrosome is disrupted in Sirt6^-/- spermatids. (B) Quantification of intensity of elongated spermatids in (A). Sirt6^+/+, 33.84±0.90; Sirt6^-/-, 27.79±0.84, 8-week mice, n=4; 150 cells were used for each group. Data are presented as mean ± SEM. *P < 0.05. (C) Quantification of disrupted elongated spermatids of the Sirt6^+/+ and Sirt6^-/- mice. Sirt6^+/+, 4.22±0.57%; Sirt6^-/-, 91.16±1.08%, 8-week mice, n=4; 200 cells were used for each group. Data are presented as mean ± SEM. ***P < 0.001.