Research Paper Volume 12, Issue 16 pp 16238—16254

Figure 8. GPNMB is a target for miR-30b-3p in STAD cells. Venn diagrams showing the number of potential miRNAs targeting the 3’UTR of GPNMB, as predicted by two databases, miRanda and miRDB. (A) The gene expression of miR-30b-3p between cancer and normal samples via ENCORI. (B) The coexpression between miR-30b-3p and GPNMB via ENCORI showed that miR-30b-3p expression is negatively correlated with GPNMB expression. (C) Sequences of miR-30b-3p and their potential binding sites in the 3’UTR of GPNMB is shown. (D) Quantitative real time PCR analyzing miR-30b-3p expression relative to MGC803 as internal control is shown. (E) Comparison of GPNMB expression in STAD cells transfected with miR-30b-3p mimic or negative control (NC) based on qRT-PCR (F), and western blotting (H), and the loading control for western blotting was GADPH. Analysis of luciferase activity from reporters containing the 3’UTR end of GPNMB in cells transfected with the miR-30b-3p mimic, miR-30b-3p mutation mimic (miR-30b-3p-mut) and negative control (NC) is shown. (G) All the above experiments were repeated six times respectively. *P < 0.05 versus control.