Research Paper Volume 12, Issue 17 pp 17114—17121
miR-345-5p regulates adipogenesis via targeting VEGF-B
Figure 1. The level of miR-345-5p was significantly downregulated during 3T3-L1 cell differentiation. (A) Oil Red O staining of lipid droplets of 3T3-L1 cells at day 0, 4, and 9 during adipocyte differentiation. (B) Real-time PCR analysis of adipocyte-specific genes (PPARg, FABP4, aP2,ADIPOQ, GLUT4 and VEGF-B) in 3T3-L1 cells during adipocyte differentiation. (C) Western blot analysis of adipocyte-specific genes (PPARg, FABP4, aP2, ADIPOQ, GLUT4 and VEGF-B). (D) Real-time PCR analysis of miR-345-5p during adipocyte differentiation. *P<0.05, **P<0.01, ***P<0.00 versus Day 0 group.