Research Paper Volume 12, Issue 17 pp 17122—17136

Figure 2. miR-21 secreted from ovarian cancer cells facilitates the secretion of IL-6 from M2 macrophages and this crosstalk elevates the expression of PD-L1 in both cell lines. (A) EVs uptake capacity of M2 macrophage visualized by CFSE-labeled EVs. (B) knock down efficiency of miR-21 assessed by RT-qPCR. (C) effect of miR-21 in EVs on the expression of IL-6 in M2 macrophage determined by Western blotting and RT-qPCR. (D) secretion of IL-6 by M2 macrophages was determined by ELISA assay. (E, F) expression of PD-L1 in M2 macrophage and SKOV3 determined by Western blotting analysis and RT-qPCR. (G) proliferation of CFSE-labeled T cells was assessed by flow cytometry. * p < 0.05 vs. control or co-M2. # p < 0,05 vs. EVs-NC. Statistical comparisons were performed using unpaired t test when only two groups were compared or by Tukey’s test-corrected one-way ANOVA with when more than two groups were compared.