Research Paper Volume 12, Issue 17 pp 17122—17136

Figure 4. SNHG12 facilitates the expression of IL-6R by NF-κB1. (A) Transcription factor for upregulation of IL-6R by SNHG12 predicted by the LNCmap database. (B) sub-localization of SNHG12 predicted by the LNCmap database. (C) sub-localization of SNHG12 visualized by FISH (D) expression of SNHG12 detected by RT- qPCR. (E) the binding of SNHG12 and NF-κB1 determined by RIP. (F) effects of SNHG12 and NF-κB1 on IL-6R promoter activity assessed by dual luciferase assay. (G) The binding of NF-κB1 to IL-6R promoter determined by ChIP. (H) the expression of IL-6R after silencing or overexpressing SNHG12 determined by Western blotting analysis. * p < 0.05 vs. control or IL-6R-WT + NC. # p < 0.05 vs. IgG. Statistical comparisons were performed using unpaired t test when only two groups were compared or by Tukey’s test-corrected one-way ANOVA with when more than two groups were compared.