Research Paper Volume 12, Issue 17 pp 17122—17136
Long non-coding RNA SNHG12 promotes immune escape of ovarian cancer cells through their crosstalk with M2 macrophages
Figure 5. SNHG12 suppresses T cell proliferation by upregulation of PD-L1. (A) The expression of miR-21 determined by RT- qPCR. (B) protein levels of PD-L1 after SNHG12 depleted SKOV3 co-cultured with M2 macrophage determined by Western blotting analysis. (C) effect of SNHG12 depletion or IL-6R overexpression in SKOV3 co-cultured with M2 macrophages, on T cells proliferation determined by flow cytometry. * p < 0.05 vs. si-NC. Statistical comparisons were performed using unpaired t test when only two groups were compared or by Tukey’s test-corrected one-way ANOVA with when more than two groups were compared.