Research Paper Volume 12, Issue 16 pp 16304—16325

Figure 2. Upregulated HOXD-AS1 facilitated the viability, migration and invasion of CCA cells. (A) The expression of HOXD-AS1 in CCLP-1, QBC939, TFK-1, RBE cells and normal HIBEC. (B) CCK-8 proliferation curves were drawn to show the impact of HOXD-AS1 on cellular proliferation. (C) EdU assay was performed to detect the effect of HOXD-AS1 on cellular proliferation. (D) The effect of HOXD-AS1 on cell colonies was revealed by colony formation assays. (E) The wound closure of CCA cells was evaluated by wound healing assays. (F) Transwell assays displayed that migrating cells were decreased in si-HOXD-AS1 cells and increased in QBC939 cells transfected with HOXD-AS1 plasmid. (G) The invasive ability of CCA cells was confirmed by transwell assay. (H) EMT-related proteins including epithelial marker (E-cadherin) and mesenchymal markers (snail and vimentin) were measured via western blot. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.