Research Paper Volume 12, Issue 16 pp 16304—16325

Figure 8. HOXD-AS1/miR-520c-3p/MYCN contributed to CCA tumorigenesis in vivo. (A) CCLP-1 cells cotransfected with sh-HOXD-AS1 and antagomir-520c-3p were subcutaneously injected into the posterior flanks of mice, and xenograft tumors were dissected on the 21^st day after injection. (B) Tumor volumes were calculated every 3 days throughout the course of tumor growth. (C) Tumor weights were measured after excision. (D) MYCN mRNA expression in xenograft tumors of the three groups (sh-NC/antagomir-NC, sh-HOXD-AS1/antagomir-NC, sh-HOXD-AS1/antagomir-520c-3p). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.