Research Paper Volume 12, Issue 16 pp 16326—16340

Figure 2. Knockdown of CD38 impedes TNF-α-induced proliferation and migration in ASM cells. (A) RT-qPCR detection was used to determine knockdown efficacy of three designed CD38 siRNAs (siCD38-331, siCD38-509 and siCD38-939; n = 3, **p < 0.01). (B) Determination of recombinant adenovirus infection efficacy via GFP fluorescence using immunocytochemistry (magnification: 200X). ASM cells were infected with Ad-CD38 shRNA or Ad-NC shRNA for 48 h and then stimulated with 20 ng × ml^-1 TNF-α for 24 h, and (C, D) protein expression of CD38 was determined via Western blot (n = 3, ***p < 0.001 and ****p < 0.0001). (E, F) The effect of CD38 knockdown on TNF-α-induced ASM cell proliferation evaluated via EdU proliferation assay (magnification: 200X) (n = 3, **p < 0.01). (G, H) The effect of CD38 knockdown on TNF-α-induced ASM cell migration assessed by Transwell migration assay (magnification: 200X) (n = 3, ***p < 0.001).