Research Paper Volume 12, Issue 16 pp 16326—16340
PTEN participates in airway remodeling of asthma by regulating CD38/Ca2+/CREB signaling
Figure 6. Knockdown of CD38 inhibits airway remodeling in asthmatic mice. (A, B) Immunohistochemistry analysis of CD38 protein expression in lung tissues. (C, D) Western blot analysis of CD38 protein expression in lung tissues (magnification: 400X). (E) Hemolysin staining of lung tissues (magnification: 400X). (F, G) Determination of ASM cell mass via immunohistochemistry staining of α-SMA (magnification: 400X). (H) Intracellular Ca2+ concentrations measured via Fluo-3 AM fluorescent probes in lung tissues. (I, J) Protein expression of total CREB an pCREB determined using Western blot. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.