Research Paper Volume 12, Issue 16 pp 16326—16340
PTEN participates in airway remodeling of asthma by regulating CD38/Ca2+/CREB signaling
Figure 9. CD38 overexpression reverses PTEN-mediated suppressive effects on airway remodeling, Ca2+ induction and CREB phosphorylation in the lung tissue of asthmatic mice. (A) HE staining of lung tissues (magnification: 400X). (B, C) Detection of ASM cell mass using immunohistochemistry staining of α-SMA (magnification: 400X). (D) Intracellular Ca2+ concentrations measured via Fluo-3 AM fluorescent probes in lung tissues. (E, F) Expression of total CREB and pCREB protein levels were determined using Western blot. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p<0.0001.