Research Paper Volume 12, Issue 18 pp 18192—18208

Figure 1. MiR-216a-3p levels correlate with MAPK14 protein expression and sorafenib sensitivity in HCC cells and tumor tissues. (A) Representative western blot images show MAPK14 protein expression in sorafenib-resistant and normal HCC cell lines. N: normal, R: resistance. GAPDH was used as loading control. (B) Q-PCR analysis shows relative MAPK14 mRNA levels in sorafenib-resistant and normal HCC cell lines. (C) Representative western blot shows MAPK14 protein expression in HCC cells transfected with miR-NC (negative control), miR3681-3p, miR128-3p and miR216a-3p mimics. GAPDH was used as loading control. (D) Pearson correlation analysis of MAPK14 protein and miR-216a-3p expression in 20 HCC patient tissue samples by IHC scores. (E) Representative western blot shows MAPK14 protein expression in tumor tissues from 3 CR (Complete response) to sorafenib and 3 NR (No response) to sorafenib HCC patients. (F) Q-PCR analysis shows relative miR-216a-3p levels in tumor tissues from 3 sorafenib-sensitive and 3 sorafenib-resistant HCC patients (n=3).