Research Paper Volume 12, Issue 18 pp 18192—18208
MicroRNA-216a-3p promotes sorafenib sensitivity in hepatocellular carcinoma by downregulating MAPK14 expression
Figure 2. MiR-216a-3p directly targets 3’UTR region of MAPK14 mRNA. (A) Schematic representation shows potential miR-216a-3p binding sites in the WT and mutated 3’UTR of MAPK14 mRNA. (B) Dual luciferase reporter assay results show the luciferase activity from WT and mutant MAPK14-3’UTR luciferase constructs in HCC cells. (C) Representative western blot and (D) histogram plot shows relative MAPK14 protein expression in HCC cells transfected with miR-NC, miR-216a-3p mimic or miR-216a-3p inhibitor. GAPDH was used as loading control.