Research Paper Volume 12, Issue 18 pp 18209—18220
piR-19166 inhibits migration and metastasis through CTTN/MMPs pathway in prostate carcinoma
Figure 8. piR-19166 inactivated CTTN /MMPs pathways in PC3 cells. (A) mRNA levels of CTTN were detected in PC3 of piR-19166 overexpressing or silencing compared with the negative control (NC) by qRT-PCR. (B) mRNA levels of MT1-MMP were detected in PC3 of piR-19166 overexpressing or silencing compared with NC by qRT-PCR. (C) Levels of MMP2 mRNA were detected in PC3 of piR-19166 overexpressing or silencing compared with NC by qRT-PCR. (D) mRNA levels of MMP9 were detected in PC3 of piR-19166 overexpressing or silencing compared with NC by qRT-PCR. (E) Protein levels of p-CTTN, p-MT1-MMP, p-MMP2 and p-MMP9 were detected in PC3 of piR-19166-overexpressing or piR-19166-knockdown compared with NC by western blot. Error bars represent the mean ± SD of three independent experiments. *P <0.05.