Research Paper Volume 12, Issue 20 pp 20139—20151
Circular RNA circ_PVT1 induces epithelial-mesenchymal transition to promote metastasis of cervical cancer
Circ_PVT1 is upregulated in cervical cancer. (A) The volcano plot of gene. (B) Heat map of microarray data was used to show the significant expression of circRNAs in tumor and normal tissue. (C) The expression of circ_PVT1 was detected in CC patients tumor tissue and normal tissues. n=43, **P<0.01. (D) RT-PCR assay was performed to measure the expression of circ_PVT1 in different CC cell lines (C33A, HCC-94, Hela and CASKI), HUCEC cell line was described as a control. n=10, *P<0.05, **P<0.01. (E) Representative photo of RNA fluorescence in situ hybridization for endogenous circ_PVT1 in C33A cells.