Research Paper Volume 12, Issue 20 pp 20139—20151
Circular RNA circ_PVT1 induces epithelial-mesenchymal transition to promote metastasis of cervical cancer
Circ_PVT1 regulated EMT in CC cells via exosome pathway. (A) The expression of circ_PVT1 was detected in exosome. n=20, *P<0.05. (B) TEM was used to determine the existence and morphology of exosomes from plasma and urine. (C) The biomarker (CD63, TSG101, HSP70 and ALIX) of exosome were detected in exosome-free media and exosome from plasma. (D) The expression of circ_PVT1 was detected in exosome from circ_PVT1/vector transfection cells. n=5, **P<0.01. (E) The expression of metastasis-associated protein, E-cadherin, Vimentin, N-cadherin and SNAIL were detected in exo-circ_PVT1/exo-vector co-cultured with C33A cells. n=5, *P<0.05.